Table 3.
Summary of disease-resistant crops developed via CRISPR/Cas9.
| Crop | Target Gene | Pathogen | Gene Function | Trait Improvement | Editing Result | Repair Pathway | Delivery Technique | Reference |
|---|---|---|---|---|---|---|---|---|
| Oryza sativa | eIF4G | Rice tungro spherical virus | Translation initiation factor | Resistance against Rice tungro spherical virus | Knock-out | NHEJ | Agrobacterium-mediated transformation | [215] |
| Vitis vinifera | VvWRKY52 | Botrytis cinerea | Transcription factor | Increased resistance against Botrytis cinerea | Knock-out | NHEJ | Agrobacterium-mediated transformation | [216] |
| Gossypium hirsutum | Gh14-3-3d | Verticillium dahliae | Negative regulator of disease resistance | Resistance to Cotton verticillium wilt | Knock-in | NHEJ | Agrobacterium-mediated transformation | [217] |
| Solanum lycopersicum | SlJAZ2 | Pseudomonas syringae | co-receptor of coronatine | Bacterial speck resistant | Knock-out | NHEJ | Agrobacterium-mediated transformation | [218] |
| Solanum lycopersicum | CP and Rep sequences | Tomato yellow leaf curl virus | Negative regulator of viral resistance | Improved resistance against Tomato yellow leaf curl virus | Knock-out | NHEJ | Agrobacterium-mediated transformation | [219] |
| Solanum lycopersicum | SlMlo1 | Oidium neolycopersici | Encoding powdery mildew resistance | Improved resistant against powdery mildew | Knock-out | NHEJ | Agrobacterium-mediated transformation | [209] |
| Triticum aestivum | EDR1 | Erysiphe cichoracearum | Encoding powdery mildew resistance | Improved resistant against powdery mildew | Knock-out | NHEJ | Particle bombardment | [208] |
| Citrus paradise | CsLOB1 | Xanthomonas citri subsp. citri | Increase susceptibility against citrus canker | Citrus canker resistant | Knock-out | NHEJ | Agrobacterium-mediated transformation | [207] |
| Citrus sinensis | CsLOB1 | Xanthomonas citri subsp. citri | Increase susceptibility against citrus canker | Citrus canker resistant | Knock-out | NHEJ | Agrobacterium-mediated transformation | [206] |
| Oryza sativa | OsERF922 | Magnaporthe oryzae | ERF transcription factor | Resistance against blast fungus | Knock-out | NHEJ | Agrobacterium-mediated transformation | [204] |
| Cucumis sativus | eIF4E | Multiple viruses | Translation initiation factor | Broad virus resistance | Knock-out | NHEJ | Agrobacterium-mediated transformation | [220] |
| Oryza sativa | OsSWEET13 | X. oryzae pv. oryzae | Sucrose transporter gene | Resistance against bacterial blight | Knock-out | NHEJ | Agrobacterium-mediated transformation | [205] |
Description: Translation initiation factor 4 gamma gene (eIF4G), WRKY transcription factor 52 (WRKY52), 14-3-3 protein 6-like (Gh14-3-3d), Jasmonate ZIM-domain protein 2 (JAZ2), Powdery mildew resistance protein (Mlo1), Enhanced disease resistance1 (EDR1), LATERAL ORGAN BOUNDARIES 1 (CsLOB1), Ethylene-responsive gene (OsERF922), Eukaryotic translation initiation factor 4E-1 (elF4E), Bidirectional sugar transporter SWEET13-like (SWEET13).