Figure 3.

Transplanted human donor and mouse host cells contribute to the bone reconstruction induced by transplantation of C-MSCs. (A) Animals were sacrificed at 1, 2, 4, and 8 weeks after surgery and the calvarial bones were fixed. Coronal sections were obtained and immunostained with anti-human Vimentin antibody (green). Nuclei (blue) were counter-stained with DAPI. Upper panels show lower magnification. Bar = 500 µm. Bottom panels are magnifications of the boxed regions. Bar = 50 µm. (B) The periphery (left and right), mid (left and right), and center in the bone defect region from each group were used for counting of human vimentin-positive and -negative cells. Results are expressed as means ± SD of the five views tested for each group. * p < 0.05, ** p < 0.01: Values differ significantly. All graphs and images are representative of four independent experiments.