Skip to main content
. 2019 Aug 14;20(16):3953. doi: 10.3390/ijms20163953

Figure 5.

Figure 5

GTN-induced anchorage-independent MDA-MB-231 cell anoikis programmed cell death. The cell viability of detachment MDA-MB-231 cell condition, cultured in a poly-HEMA coated plate were compared to MCF-10A cells as mean ± SD, which was determined by the MTT assay (A). Matrix metalloproteinase activity; MMP-2 and MMP-9 were investigated by gelatin zymography (B). The bar graphs illustrate the mean ± SD of the average band intensity from three independent experiments of gelatin zymography of MMP-9 (C), Pro MMP-2 (D), and MMP-2 (E). The epithelial-mesenchymal transition (EMT) proteins and the expressions of cell survival molecules, EGFR, FAK, and Src, and the BH3-only protein, Bim-EL, were demonstrated by Western blotting (F). The bar graphs illustrated are the mean ± SD of the band density from three independent experiments of Western blotting (G). The significance of statistical values from three independent experiments are marked with * p < 0.05; ** p < 0.01 when compared with the un-treated detached cells and those compared between un-treated groups (adherent cells and detachment cells) were marked with # p <0.05; ## p < 0.01.