Figure 1.

(a) The proliferation of human mesenchymal stem cells (hMSCs)with osteogenic medium under different culture environments was determined based on total DNA quantification, and the flow reactor had a large cell number relative to static-state culture and spinner flask; (b) the mitochondria activity of hMSCs under variant culture system was determined by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and cells in the flow reactor had a relatively higher viability (*p < 0.05).