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. 2019 Sep 2;20:448. doi: 10.1186/s12859-019-3055-3

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Cell diameter recovery workflow as annotated Explorer screenshots. a Unstimulated/naive cells with DAPI stain (gray), binary PHA image (red), and resulting cell segmentation (green). b Projection of modes in phalloidin/DAPI distribution to segmented cells in original image. c Diameter distribution comparison for unstimulated and activated samples along with corresponding single cell images