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. 2019 Aug 16;20(16):4006. doi: 10.3390/ijms20164006

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Kinetics of F_min. The final assay procedure was performed up to the measurement of F as described in Materials and Methods and the experiment was started by addition of either (A) ethylenediaminetetraacetic acid (EDTA) or (B) N,N,N′,N′-tetrakis(2-pyridinylmethyl)-1,2-ethanediamine (TPEN) solution (final concentrations as indicated). Fluorescence was measured in 1 min intervals for a total duration of 60 min. Data are shown relative to the value for F used in the final assay procedure, representing means of at least n = 3 independent experiments.