Table 5.
Activity of total superoxide dismutase (SOD), Cu/Zn superoxide dismutase (Cu/Zn SOD) and alkaline phosphatase (ALP) in tissues of piglets fed diets differing in zinc and fibre source.
| Enzyme Activity | Dietary Treatment | SEM | Main Effects | Interaction | ||||
|---|---|---|---|---|---|---|---|---|
| C | ZnGly | PF | PF + ZnGly | Zn | Fibre | Zn × Fibre | ||
| Total SOD, U/mg protein | ||||||||
| Liver | 24.1 | 23.5 | 28.9 | 25.9 | 0.782 | 0.27 | 0.05 | 0.25 |
| Kidney | 7.67 | 7.91 | 6.60 | 8.84 | 0.428 | 0.16 | 0.93 | 0.25 |
| Pancreas | 8.70 | 6.24 | 7.94 | 7.40 | 0.612 | 0.25 | 0.88 | 0.45 |
| Duodenum | 9.93 a | 13.5 b | 12.7 ab | 9.61 a | 0.636 | 0.85 | 0.64 | 0.01 |
| Jejunum | 5.72 | 7.39 | 7.13 | 7.32 | 0.376 | 0.24 | 0.40 | 0.35 |
| Cu/Zn SOD, U/mg protein | ||||||||
| Liver | 21.6 | 21.6 | 26.3 | 22.9 | 0.843 | 0.25 | 0.03 | 0.46 |
| Kidney | 6.49 | 6.58 | 5.50 | 7.49 | 0.385 | 0.19 | 0.96 | 0.23 |
| Pancreas | 5.73 | 4.12 | 4.73 | 4.20 | 0.468 | 0.28 | 0.64 | 0.58 |
| Duodenum | 5.65 | 8.13 | 7.22 | 5.89 | 0.457 | 0.67 | 0.88 | 0.02 |
| Jejunum | 3.44 | 4.77 | 3.92 | 4.49 | 0.268 | 0.09 | 0.86 | 0.49 |
| ALP 1 | ||||||||
| Duodenum | 14.7 | 14.0 | 13.6 | 15.2 | 0.353 | 0.36 | 0.93 | 0.06 |
| Jejunum | 12.2 | 12.0 | 12.3 | 12.8 | 0.253 | 0.80 | 0.44 | 0.49 |
C: cellulose, ZnGly: zinc chelate of glycine hydrate, PF: potato fibre. a,b Means within lines with different superscript letters are significantly different (p < 0.05) using Fisher’s Least Significant Difference (LSD) post hoc test. 1 The enzyme activity is given as density values (pixel intensities) in duodenal and jejunal enterocytes at a wavelength of 520 nm; Bold values denote statistical significance at the p < 0.05.