Figure 3.

The uptake of manganese (Mn) from MnSO₄ and Mn-lysine (MnLys) complex in the primary chicken intestinal epithelial cells (IEC). (A) Effect of iron (Fe) addition on the uptake of Mn from different Mn sources. The cells were treated with 0 (control) or 1.50 mM Fe (Fe addition) and 0.25 mM MnSO₄ or MnLys for 60 min. (B) Influences of N-ethylmaleimide (NEM) on the uptake of Mn from different Mn sources. The cells were pretreated with 5 μM NEM for 10 min and then were treated with 0.25 mM MnSO₄ or MnLys for 60 min in the presence of NEM. (C) Influences of cycloheximide (CHX) on the uptake of Mn from different Mn sources. The cells were pretreated with 5 μM CHX for 10 min and then were treated with 0.25 mM MnSO₄ or MnLys for 60 min in the presence of CHX. Interaction of Mn source and Fe, NEM, or CHX treatment influenced (p < 0.05) Mn uptake in primary chicken IECs. Values are least-square means ± standard error (n = 8). ^a–c Mean values lacking a common letter are different at p < 0.05.