Figure 4.

Influences of Fe addition and Mn source on ionized Mn²⁺ transporters expression in primary chicken intestinal epithelial cells (IECs). The primary confluent IECs were incubated with 0 or 1.50 mM Fe (ferric ammonium citrate), and 0.25 mM Mn from MnSO₄ or Mn-lysine complex (MnLys) for 60 min. The mRNA abundance of divalent metal transporter 1 (DMT1) (A) and Figure 1. (FPN1) (B) were determined by real-time RT-PCR. The Fe addition affected DMT1 mRNA abundance, and Mn source and Fe addition influenced FPN1 transcriptional expression (p < 0.05). Values are least-square means ± standard error (n = 8). ^a–c Mean values lacking a common letter are different at p < 0.05.