Figure 5.

Influences of N-ethylmaleimide (NEM) and Mn source treatments on the membrane transporters genes expression in the primary chicken intestinal epithelial cells (IECs). The primary confluent IECs were pretreated with 5 μM NEM for 10 min and then were incubated with 0.25 mM Mn from MnSO₄ or Mn-lysine complex (MnLys) for 60 min in the presence of NEM. The mRNA abundance of cationic amino acid transporter (CAT) 1, CAT2, divalent metal transporter 1 (DMT1), and ferroportin 1 (FPN1) was determined by real-time RT-PCR. Interaction of Mn source and NEM addition influenced CAT1, CAT2, and FPN1 transcriptional expression (p < 0.05). Values are least-square means ± standard error (n = 8). ^a–d Mean values lacking a common letter are different at p < 0.05.