Figure 6.

Influences of cycloheximide (CHX) and Mn source treatments on the membrane transporters genes expression in the primary chicken intestinal epithelial cells (IECs). The primary confluent IECs were pretreated with 10 μM CHX for 10 min and then were incubated with 0.25 mM Mn as MnSO₄ or Mn-lysine complex (MnLys) for 60 min in the presence of CHX. The mRNA abundance of transport system b^0,+ components rBAT and b^0,+AT, and ionized Mn transporters divalent metal transporter 1 (DMT1), and ferroportin 1 (FPN1) was determined by real-time RT-PCR. Interaction of Mn source and CHX treatment influenced rBAT and b^0,+AT expression but only the Mn source affected the FPN1 mRNA abundance (p < 0.05). Values are least-square means ± standard error (n = 8). ^a–d Mean values lacking a common letter are different at p < 0.05.