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. 2019 Aug 11;20(16):3911. doi: 10.3390/ijms20163911

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effect on translation of the first nucleotide downstream the wild-type pseudo knot (PKI). (A) Histogram representing the luciferase activities of the synthesized R-Luc in 0-frame or +1-frame. Translation efficiencies are represented as raw bioluminescence activity (Relative Light Units or RLU) and resulted from at least 3 independent experiments. Standard deviations are shown. The construct with a G+1 corresponds to the WT PKI. (B) The same data represented as a 100% stack bar graph.