Figure 7.

The effect on translation of reprogramming the PKI with different Gly codon-anticodon combinations. (A) Schematic representations of the different RNAs tested. Non Watson-Crick base pairs are highlighted in yellow. (B) Histogram representing the luciferase activities of the synthesized R-Luc in 0-frame or +1-frame. Translation efficiencies are represented as raw bioluminescence activity (Relative Light Units or RLU) and resulted from at least 3 independent experiments. Standard deviations are shown. Construct names are described as XXX/YYY-G where X represent anticodon triplets, Y codon triplets, all with a G in +1 and written in the 5′-3′ direction. The first construct corresponds to the WT PKI.