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T cell proliferation assay and release of cytokines. Lymph node T cell proliferation was measured by bromdeoxiuridine incorporation. O.D. (optical density, 405 nm). ELISA measured levels of T cell differentiation cytokines in the conditioned media of the cell proliferation assay. Data are presented as mean ± SEM (n = 5), one-way ANOVA with Tukey’s post-test. # p < 0.05, ## p < 0.01, ### p < 0.001 versus naïve group; * p < 0.05, ** p < 0.01, *** p < 0.001 versus control group.
