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. 2019 Aug 7;20(16):3845. doi: 10.3390/ijms20163845

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Bone density and osteoclast area analysis. (A) X-rays analysis of both front and hind limbs. Bone density was measured by analyzing a ROI (region of interest) around the wrist and ankle joints. Values are presented in Hounsfield units. Representative images of hind paws with color scale: red, high bone density; green/yellow, middle density; blue, low density (n= 5). (B) Tartrate-resistant acid phosphatase (TRAP) staining of mice ankle sections. The purple staining represent the TRAP positive area associated to osteoclasts activity. Bar = 200 μm and 50 μm. Data are presented as mean ± SEM (n = 5). One-way ANOVA with Tukey’s post-test. # p < 0.05, ### p < 0.001 versus naïve group; * p < 0.05, *** p < 0.001 versus control group.