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. 2019 Aug 10;19(16):3502. doi: 10.3390/s19163502

Figure 2.

Figure 2

Protein characterization of the mScarlet-ε-NanoLuc sensor called ARSeNL (ATP detection with a Ratiometric mScarlet-NanoLuc sensor). (A) Luminescence emission spectra and (B) the ATP-dependent mScarlet/NanoLuc BRET ratio response (KD = 1.1 ± 0.1 mM, n = 3) were measured on a microplate reader, and data were fitted to the Hill equation. (C,D) The BRET ratio (purple) is stable over time, even while the total luminescence intensity (blue) decays rapidly. The BRET ratio is (C) 0.0063 ± 0.0001 in the presence of 0 mM ATP and (D) 0.0324 ± 0.009 in the presence of 10 mM ATP (n = 3).