Table 1.
Library Screening Results.
| Donor (Terminus) | Acceptor (Terminus) | Fold-Change in Ratio | |
|---|---|---|---|
| FRET | BRET | ||
| NanoLuc (C) | mScarlet (N) | na ‡ | 5.12 ± 0.18 |
| NanoLuc (C) | tdTomato (N) | na | 4.83 ± 0.20 |
| NanoLuc (C) | GRvT (N) | na | 4.56 ± 0.29 |
| NanoLuc (N) | mScarlet (C) | na | 1.07 ± 0.21 |
| NanoLuc (N) | tdTomato (C) | na | - * |
| NanoLuc (N) | GRvT (C) | na | - * |
| GeNL 1 (C) | mScarlet (N) | 1.53 ± 0.01 | 1.90 ± 0.02 |
| GeNL (C) | tdTomato (N) | - * | - * |
| GeNL (C) | RRvT (N) | 1.39 ± 0.01 | 2.04 ± 0.01 |
| CeNL 2 (C) | mScarlet (N) | 1.88 ± 0.01 | 1.96 ± 0.01 |
| CeNL (C) | tdTomato (N) | - * | - * |
| CeNL (C) | RRvT (N) | 1.50 ± 0.01 | 2.07 ± 0.02 |
| CeNL (C) | mNeonGreen (N) | 1.86 ± 0.06 | 1.25 ± 0.02 |
‡ Not applicable to BRET-only sensors. * Poor or no expression. 1 GeNL is mNeonGreen-NanoLuc. 2 CeNL is mTurquoise2-NanoLuc. BRET: bioluminescence resonance energy transfer; FRET: Förster-type resonance energy transfer.