Figure 11.

The influence of 24 h exposure of V. opulus juice (FJ), PRF (phenolic rich fraction), MAE (methanol-acetone extract) and AE (acetone extract) at 50 µg/mL on Caco-2 cells in the presence of 100 µM palmitic acid on the cellular lipid accumulation obtained with Nile red assay (A). The influence of 24 h exposure of extracts on fatty acid analogue TF2-C12 uptake measured with FFA-uptake assay (B). The influence of the PRF and MAE extracts on the accumulation of lipid droplets in Caco-2 cells stained with Nile red was observed by treating cells with the extracts for 24 h in the presence of palmitic acid (100 µM). High fluorescence in positive control cells after incubation with palmitic acid was observed. Fluorescence microscopy (Nikon TS100 Eclipse, Japan), 200× magnification. Images are representative of one of four similar experiments (C). Control cells were not exposed to any compound but the vehicle; values are means ± standard deviations from at least three independent experiments; statistical significance was calculated versus control cells (untreated) * p ≤ 0.05, ** p ≤ 0.01.