Figure 3.

Effect of HUVECs pretreated with TH on H₂O₂-induced cytoskeletal remodeling and gap formation. Actin cytoskeleton (green) is shown merged with its nucleus (blue) stained with DAPI. Representative images were taken from one of three independent experiments at 400x magnification. Merged images were generated with Olympus BX-51 Cell-F imaging software. Images represent basal (a); H₂O₂ at 0.5 mM (b); and pretreatments+inducer: TH 0.01%+H₂O₂ (c), TH 0.10%+H₂O₂ (d), TH 1.00%+H₂O₂ (e), and TR+H₂O₂ (f). Basal = untreated; TH = Tualang honey; TR = Trolox.