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. 2019 Aug 7;20(16):3852. doi: 10.3390/ijms20163852

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Assessment of in vitro photodynamic treatment (PDT). (a) The cells were pre-incubated for 30 min with liposomal formulation of Photolon and irradiated with a 655 nm diode laser for the indicated time. The viability of the cell cultures was assayed using a MTS assay. Results are expressed as a percentage of untreated control. Average ± SD are shown. (b) Irradiation-induced reactive oxygen species (ROS) assayed with a fluorescent H₂DCFDA probe. Average RLU ± SD are shown. (c) Morphology and density of irradiated J774A.1 cell cultures subjected to photosensitization.

Assessment of in vitro photodynamic treatment (PDT). (a) The cells were pre-incubated for 30 min with liposomal formulation of Photolon and irradiated with a 655 nm diode laser for the indicated time. The viability of the cell cultures was assayed using a MTS assay. Results are expressed as a percentage of untreated control. Average ± SD are shown. (b) Irradiation-induced reactive oxygen species (ROS) assayed with a fluorescent H₂DCFDA probe. Average RLU ± SD are shown. (c) Morphology and density of irradiated J774A.1 cell cultures subjected to photosensitization.