Figure 3.

Rat Muller cells (rMC-1) were exposed to hypoxia for 1hour followed by reperfusion in normoxia for 24-hours. Statistical analysis of mRNA level assessed by realtime-PCR showed a significant increase in ER-stress marker IRE-1α (a), decrease in miR-17-5p (b), Marked increase (20-fold) in TXNIP mRNA (c) compared to cells grown in normoxia. (p < 0.05 vs. normoxia, N = 4–6). Deletion of TXNIP blunted the increase in IRE-1α (d), restored miR-17-5p and increase in TXNIP (f) in response to IR when compared to WT (N = 4–6, * p < 0.05, two-way ANOVA two-way ANOVA showed significant effect of IR and gene deletion).