rHc-HCA59	Recombinant Hepatocellular Carcinoma-associated antigen 59
PI	Post infection
HcESPs	Excretory and secretory products of Haemonchus contortus
NAU	Nanjing Agricultural University
LB	Luria Bertini
AEC	Animal Ethics Committee
IPTG	Isopropyl-b-D-thiogalactopyranoside
SDS-PAGE	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis
PVDF	Polyvinyl difluoride membrane
HRP	Horseradish peroxidase
TMB	Tetramethylbenzidine
OD	Optical density
P/N	Positive to negative
BSA	Bovine serum albumin
TBS-T	Tris-Buffered Saline containing 0.05% Tween 20
MW	Molecular weight
kDa	Kilodalton
PBMCs	Peripheral blood mononuclear cells