Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 11;20(16):3907. doi: 10.3390/ijms20163907

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Derivative curve high-resolution melt profiles and mean methylation output ratios of CpG sites located within the Fragile X-Related Epigenetic Element 2 (FREE2) region assessed using Methylation-Specific Quantitative Melt Analysis (MS-QMA) between different tissues for (A) B1 and (B) B2. (C) DNA samples from lymphoblasts of a fragile X syndrome (FXS) male with the fragile X mental retardation 1 (FMR1) promoter 100% methylated spiked with DNA from a typically developing control (cytosine-guanine-guanine (CGG) < 44) with the FMR1 promoter 0% methylated. These samples were mixed at different ratios for the MS-QMA methylation reference curve, with the expected % of methylation indicated on the plot for each derivative curve profile.