Table 2.

Results of full factorial design experiments for the extraction of silymarin (SILM) from mature fruits of S. marianum.

Run ID	Run Order	X1	X2	X3	SILM (mg/g DW)
Run ID#1	17	−1	−1	−1	2.24 ± 1.80
Run ID#2	24	0	−1	−1	6.99 ± 4.11
Run ID#3	26	+1	−1	−1	4.33 ± 3.09
Run ID#4	21	−1	0	−1	2.55 ± 4.61
Run ID#5	22	0	0	−1	9.66 ± 1.44
Run ID#6	6	+1	0	−1	6.51 ± 3.06
Run ID#7	10	−1	+1	−1	4.37 ± 1.72
Run ID#8	27	0	+1	−1	8.45 ± 4.48
Run ID#9	7	+1	+1	−1	6.88 ± 4.82
Run ID#10	18	−1	−1	0	3.21 ± 0.34
Run ID#11	12	0	−1	0	12.12 ± 1.57
Run ID#12	8	+1	−1	0	6.15 ± 1.08
Run ID#13	25	−1	0	0	1.80 ± 1.50
Run ID#14	1	0	0	0	14.25 ± 0.51
Run ID#15	16	+1	0	0	9.79 ± 2.47
Run ID#16	23	−1	+1	0	2.49 ± 1.59
Run ID#17	11	0	+1	0	12.88 ± 3.35
Run ID#18	14	+1	+1	0	9.13 ± 1.80
Run ID#19	15	−1	−1	+1	2.49 ± 0.33
Run ID#20	3	0	−1	+1	16.00 ± 0.35
Run ID#21	13	+1	−1	+1	7.27 ± 0.36
Run ID#22	9	−1	0	+1	4.20 ± 0.30
Run ID#23	5	0	0	+1	16.71 ± 0.19
Run ID#24	19	+1	0	+1	10.05 ± 0.48
Run ID#25	4	−1	+1	+1	4.04 ± 0.30
Run ID#26 *	20	0	+1	+1	17.98 ± 0.66
Run ID#27	2	+1	+1	+1	8.54 ± 0.94

Values are the mean ± RSD of 3 independent replicates except for *, which correspond to the highest SILM content here determined by 6 independent experiments to confirm this value.