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. 2019 Aug 20;20(16):4054. doi: 10.3390/ijms20164054

Figure 4.

Figure 4

T1AM and SG-2 treatments are associated with decreased lipid accumulation. 3T3-L1 adipocytes were treated with T1AM or SG-2 (1, 2, 5, 10 µM) at the beginning of differentiation (day 0) (A) Visible decreases in lipid accumulation were readily observable at day 5 with SG-2 treatment. Treatment with T1AM did not result in visibly detectible decreases in lipid accumulation (pictures not shown). (B) Fluorescent intensity of Nile Red lipid stain in 3T3-L1 differentiated adipocytes treated with 20 µM T1AM and 10 µM SG-2 and measured by flow cytometry. Nile red is a fluorescent stain that integrates into and fluoresces when in contact with lipid, allowing for measured fluorescent intensity to correspond to the degree of lipid present in cells. Flow cytometry data are presented as mean channel fluorescence, with +/− SEM. * denotes a significant difference from control at p < 0.05 (ANOVA Dunnett’s test).