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. 2019 Jul 26;11(8):1058. doi: 10.3390/cancers11081058

Figure 1.

Figure 1

MDA-MB-231-ALDH1A1/tdTomato cancer stem cell (CSC) model. After transfection with reporter vector ALDH1A1/tdTomato, CSC-like cells express fluorescent reporter (tdTomato) under the CSC-specific promoter (ALDH1A1). Scale bar represents 20 µm (A). This allows the CSC quantification and sorting by fluorescence-activated cell sorting (FACS) (B). Sorted tdTomato+ cell population dropped and stabilized over passages (C). We confirmed by qPCR, that tdTomato+ cells (CSC) express stem cell markers. Results are expressed as NRQ (relative normalized quantities) mean ± SEM (n ≥ 3); * p < 0.05; ** p < 0.01, *** p < 0.001 (D).