Figure 10.

Depletion of blast cells from whole PBMCs. PBMCs from a patient with CML were incubated or not with the biotinylated NW peptide (10 µg/mL), followed by streptavidin-conjugated magnetic beads, as described in Materials and Methods. After magnetic separation, non-binding cells were analyzed by flow cytometry to verify blast depletion. 15,000 events were recorded for each sample.