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. 2019 Sep 2;18:168. doi: 10.1186/s12944-019-1103-7

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 11 — Effect of 4-cholesten-3-one on the expression of Flotillin-2 and EGFR in membrane raft fractions. Membrane rafts were isolated from MCF-7 and MDA-MB-231 cells as described in the Materials and Methods section. The membrane raft fractions were then collected, separated by SDS-PAGE and analyzed by western blot using anti-Flotillin-2 and anti-EGFR antibodies. A representative western blot showing Flotillin-2 and EGFR expression in cancer cells treated (+) or not treated (−) with 4-cholesten-3-one