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. 2019 Aug 1;11(8):1094. doi: 10.3390/cancers11081094

Figure 3.

Figure 3

Small compounds activate microautophagy in cancer cells. (a) Western blot of proteins extracted from MDA-MB-468 cells after transfection with EGFR siRNAs or scrambled siRNAs and exposure to 25 µM VM25 and VM26 for 2 h in serum-deprived Dulbecco’s Modified Eagle Medium (DMEM). (b) Relative levels of proteins were estimated as the ratio to β-actin and adjusted to the vehicle-treated cells normalized to 100%. (c) Immunofluorescence detection of LC3α/β in MDA-MB-468 cells exposed to 2.5 µM VM26 for 1 h. The size bar is equal to 10 μm.