Figure 4.

EGFR rapidly responds to the chemical invasion of small compounds in cancer cells. Immunofluorescence detection was carried out with anti-EGFR mAb (the epitope in the extracellular domain). Fluorescence in nonpermeabilized (a) and permeabilized (b) cells after exposure to vehicle and in permeabilized cells after exposure to VM23 (c) and VM26 (d). MDA-MB-468 cells were incubated with VM23 and VM26 as described in Figure 3c. Profiling curves of fluorescent signal distribution in representative cells are shown with dashes in merge. (e) Boxplot histogram of a 2D square of nuclei surfaces upon exposure to vehicle and VM26. Sav is the average square of the nuclear surface in cells. The size bar is equal to 10 μm.