Figure 6.

Effect of atezolizumab on MDA-MB-231 cell death/apoptosis, proliferation, and viability. MDA-MB-231 cells were cultured without or with 0.5 µg/mL of human IgG1 (isotype control) or atezolizumab for five days. Representative flow cytometric plots show the percentage of apoptotic cells and necrotic cells (A). Overall percentages of apoptotic and necrotic cells (non-treated (NT), IgG1- or atezolizumab-treated cells (T)) (B). Percentage in cell proliferation using a BrdU assay (C). Absorbance readings for MTT in MDA-MB-231 cells (D). Results are from two independent experiments (three–six replicates), and they are expressed as the mean ± SEM. Paired t-test, * p < 0.05, ** p < 0.01.