Table 2.
Comparison of 59 patients for whom HLA expression (mRNA), chromosome 3 status, and type of GNAQ/GNA11 mutation was known. Significant values are shown in bold. The categorical groups were compared using a chi-squared test and numerical data was compared using an independent t-test.
| Characteristics | D3 Q209L (n = 15) |
D3 Q209P (n = 6) |
M3 Q209L (n = 27) |
M3 Q209P (n = 11) |
Disomy 3 Tumors Q209L versus Q209P p-Value |
Monosomy 3 Tumors Q209L versus Q209P p-Value |
Monosomy 3 versus Disomy 3 p-Value |
|---|---|---|---|---|---|---|---|
| Gender n (%) | 0.48 1 | 0.96 1 | 0.21 1 | ||||
| Female | 5 (33) | 3 (50) | 15 (56) | 6 (55) | |||
| Male | 10 (66) | 3 (50) | 12 (44) | 5 (45) | |||
| Age at enucleation | 0.92 2 | 0.70 2 | 0.15 2 | ||||
| Mean | 53.78 | 54.64 | 64.19 | 66.01 | |||
| ±St. dev. | ± 19.21 | ±15.39 | ±14.20 | ±12.23 | |||
| Affected eye n (%) | 0.48 1 | 0.39 1 | 0.28 1 | ||||
| Right eye | 5 (33) | 3 (50) | 13 (48) | 7 (64) | |||
| Left eye | 10 (66) | 3 (50) | 14 (52) | 4 (36) | |||
| Survival n (%) | 0.88 1 | 0.75 1 | 0.001 1 | ||||
| Alive | 7 (47) | 3 (50) | 3 (11) | 2 (18) | |||
| Cause of Death: | |||||||
| Melanoma-related | 4 (27) | 1 (17) | 22 (81) | 8 (73) | |||
| Different cause | 3 (20) | 1 (17) | 1 (4) | 1 (9) | |||
| Unknown | 1 (7) | 1 (17) | 1 (4) | 0 (0) | |||
| Metastases n (%) | 0.63 1 | 0.55 1 | <0.001 1 | ||||
| No | 11 (73) | 5 (83) | 5 (19) | 3 (27) | |||
| Yes | 4 (27) | 1 (17) | 22 (81) | 8 (73) | |||
| Basal diameter | 0.34 2 | 0.27 2 | 0.30 2 | ||||
| Mean (mm) | 13.2 | 11.83 | 13.56 | 15.55 | |||
| ±St. dev. | ±2.6 | ±2.9 | ±3.3 | ±5.3 | |||
| Prominence | 0.67 2 | 0.56 2 | 0.86 2 | ||||
| Mean (mm) | 6.97 | 7.67 | 7.80 | 8.36 | |||
| ±St. dev. | ±2.7 | ±3.5 | ±2.8 | ±2.7 | |||
| Mitotic count | 0.29 2 | 0.22 2 | 0.96 2 | ||||
| Mean 3 | 7.20 | 4.67 | 5.81 | 9.64 | |||
| ±St. dev. | ±5.38 | ±4.41 | ±3.37 | ±9.39 | |||
|
Ciliary body
involvement n (%) |
0.24 1 | 0.13 1 | 0.011 1 | ||||
| No | 12 (80) | 6 (100) | 10 (37) | 8 (73) | |||
| Yes | 3 (20) | 0 (0) | 17 (63) | 3 (27) | |||
| Cell type | 0.78 1 | 0.70 1 | 0.043 1 | ||||
| Spindle | 8 (53) | 3 (50) | 5 (19) | 3 (27) | |||
| Mixed | 6 (40) | 3 (50) | 17 (63) | 7 (64) | |||
| Epithelioid | 1 (7) | 0 (0) | 5 (19) | 1 (9) | |||
| Extra-ocular extension | - | 0.12 1 | 0.67 1 | ||||
| <5 mm | 1 (50) | 0 (0) | 4 (80) | 0 (0) | |||
| >5 mm | 1 (50) | 0 (0) | 1 (20) | 1 (100) | |||
|
Chr. 8q status
n (%) |
0.16 1 | 0.64 1 | <0.001 1 | ||||
| Normal | 10 (66) | 2 (33) | 4 (15) | 1 (9) | |||
| Aberrant | 5 (33) | 4 (67) | 23 (85) | 10 (91) | |||
| Chr. 6 status n (%) | 0.24 1 | 0.80 1 | <0.001 1 | ||||
| Normal | 3 (20) | 0 (0) | 23 (85) | 9 (82) | |||
| Aberrant | 12 (80) | 6 (100) | 4 (15) | 2 (18) |
1 Pearson’s chi-squared test. 2 Two-tailed t-test (without equal variances). 3 mitosis/2 mm2. Evaluating the group sizes for the different groups in Table 2 with a Fisher’s exact test yielded no significance either (p-value = 0.235). This means that the ratio of M3/D3 patients is not significantly different between the type of mutation.