Skip to main content
. 2019 Jul 31;8(8):802. doi: 10.3390/cells8080802

Figure 1.

Figure 1

The expression of cyclin-dependent kinase (CDK) inhibitors, p15, p16, and p21, are downregulated under hypoxia. (A) Expression levels of CDK inhibitors were compared in PASMCs exposed to normoxia or hypoxia by next generation sequencing (NGS)-based RNA sequencing. (B) mRNA levels of CDK inhibitors normalized to 18S rRNA were examined by qRT-PCR in PASMCs exposed to normoxia or hypoxia for 24 h. Data represent the mean ± S.E. of triplicates. * p < 0.05. (C) Total cell lysates from pulmonary artery smooth muscle cells (PASMCs) exposed to normoxia or hypoxia for 24 h were subjected to immunoblot analysis with antibodies against p15, p16, p21, or β-actin. By densitometry, relative amounts of indicated proteins normalized to β-actin were quantitated. * p < 0.05. (D) PASMCs were exposed to normoxia or hypoxia in the absence or presence of actinomycin D and their transcript levels of p15, p16, and p21 normalized to 18S rRNA were examined by qRT-PCR. * p < 0.05.