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. 2019 Jul 31;8(8):802. doi: 10.3390/cells8080802

Figure 2.

Figure 2

Hypoxia-induced miRNA candidates that regulate CDK inhibitors. (A) Expression levels of miRNAs were compared in PASMCs exposed to normoxia or hypoxia by NGS-based small RNA sequencing. (B) PASMCs were transfected with control mimic, miR-497, or miR-665 for 24 h and subjected to qRT-PCR analysis of p15. The relative levels of mRNA expression normalized to 18S rRNA were quantitated. Data represent the mean ± S.E. of triplicates. * p< 0.05. (C) Levels of endogenous p16 mRNA relative to 18S rRNA were quantified by qRT-PCR analysis in PASMCs transfected with control mimic, miR-410-3p, or miR-1268a. * p < 0.05. (D) Levels of p21 mRNA relative to 18S rRNA were quantified by qRT-PCR analysis in PASMCs transfected with control mimic, miR-132-3p, miR-212-3p, or miR-665. * p < 0.05. (E) Expression levels of miR-497, miR-1268a, and miR-665 normalized to U6 snRNA were examined by qRT-PCR in PASMCs exposed to normoxia or hypoxia for 24 h. * p < 0.05.