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. 2019 Jul 31;8(8):802. doi: 10.3390/cells8080802

Figure 3.

Figure 3

Identification of miRNAs that regulate endogenous levels of CDK inhibitors. (A) Total cell lysates from PASMCs transfected with control mimic, miR-497, miR-1268a, or miR-665 for 24 h were subjected to immunoblot analysis with antibodies against p15, p16, p21, or β-actin. By densitometry, relative amounts of CDK inhibitor (CKI) proteins normalized to β-actin were quantitated. * p < 0.05. (B,C) Expression levels of miR-497, miR-1268a, and miR-665 normalized to U6 snRNA were examined by qRT-PCR in PASMCs transfected with control mimic, miR-497, miR-1268a, miR-665, anti-miR-497, anti-miR-1268a, or anti-miR-665. * p < 0.05. (D) Levels of endogenous p15, p16, or p21 mRNAs relative to 18S rRNA were quantified by qRT-PCR analysis in PASMCs transfected with control mimic, anti-miR-497, anti-miR-1268a, or anti-miR-665. * p < 0.05. (E) Total cell lysates from PASMCs transfected with control mimic, anti-miR-497, anti-miR-1268a, or anti-miR-665 were subjected to immunoblot analysis with antibody against p15, p16, p21, or β-actin. By densitometry, relative amounts of CKI proteins normalized to β-actin were quantitated. * p < 0.05.