miR-497, miR-1268a, or miR-665 promotes the proliferation of vascular smooth muscle cells (VSMCs). (A,B) Representative images of Ki-67 immunostaining of PASMCs transfected with control, miR-497, miR-1268a, miR-665, anti-miR-497, anti-miR-1268a, or anti-miR-665, and calculation of Ki-67 index. Approximately, 200 cells from at least 10 independent fields were counted for each condition, and Ki-67 positive cells are presented as a percentage of the total population. Scale bar represents 20 μ. * p < 0.05. (C,D) Luminescent signals for viable PASMCs transfected with control, miR-497, miR-1268a, miR-665, anti-miR-497, anti-miR-1268a, or anti-miR-665 for 3 days. * p < 0.01.