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. 2019 Aug 17;11(8):1201. doi: 10.3390/cancers11081201

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Hydroxyprogesterone (OHPg)-treated breast cancer cells show low motility, migration and invasion. (A) T47-D phalloidin staining of F-actin (stress fibers, red). 4′,6-diamidino-2-phenylindole (DAPI), nuclear staining. (B,C) Wound-healing assay (insets: time 0). T47-D and MCF-7 cells were transfected with non specific (NS) or targeted against Progesterone-Receptor (PR)-B siRNA. MDA-MB-231 were transfected with vector control (VC) or progesterone receptor B (PR-B) expression vector. (D) Transmigration assay, (E) Invasion assay. Columns are the mean of three independent experiments each in triplicate; bars, SD; * p ≤ 0.05 vs. vehicle treated cells. ** p ≤ 0.05 vs. OHPg-treated cells.

Hydroxyprogesterone (OHPg)-treated breast cancer cells show low motility, migration and invasion. (A) T47-D phalloidin staining of F-actin (stress fibers, red). 4′,6-diamidino-2-phenylindole (DAPI), nuclear staining. (B,C) Wound-healing assay (insets: time 0). T47-D and MCF-7 cells were transfected with non specific (NS) or targeted against Progesterone-Receptor (PR)-B siRNA. MDA-MB-231 were transfected with vector control (VC) or progesterone receptor B (PR-B) expression vector. (D) Transmigration assay, (E) Invasion assay. Columns are the mean of three independent experiments each in triplicate; bars, SD; * p ≤ 0.05 vs. vehicle treated cells. ** p ≤ 0.05 vs. OHPg-treated cells.