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HLA-typed Uveal melanoma (UM) cell lines can be analysed by Fluorescence-Activated Cell Sorting (FACS) using HLA antigen specific monoclonal antibodies to find defects in the expression of HLA alleles, and to determine the sensitivity of induction by Interferon gamma (IFNγ). Loss of HLA Class I allele expression in UM cell lines: B7 and B52 loss in Mel270, B27 and B40 loss in OMM1, B44 loss in 92.1. (** p = 0.01; *** p = 0.001).
