Figure 1.

Effects of Isoliquiritigenin (ISL) on the cell viability of uterine leiomyoma ELT3 cells and primary uterine smooth muscle (UtSMC) cells. (A,B) ELT3 and UtSMC cells were seeded in 96-well plates (2500 cells per well) with 100 μL per well culture medium. After cell attachment to the bottom of well, both cell types were treated with ISL in various doses for 24 and 48 h. At the end of incubation, cell viability was measured by the MTT assay (n = 4). (C,D) ELT3 (1.8 × 10⁴ cells per well) and UtSMC (2.5 × 10⁴ cells per well) cells were seeded in 24-well plates. Both cell types were treated with various doses of ISL for 24 and 48 h. Cell viability was detected using the crystal violet assay (n = 4). (E–G) ELT3 (6 × 10⁴ cells per well) and UtSMC (1.5 × 10⁵ cells per well) cells were seeded in the 6-well plates. Both cell types were treated with ISL in various doses for 24 and 48 h. Cell morphology was photographed and cell numbers were counted using trypan blue stain and an automatic cell counter (n = 3). (magnification 100×; Scale bar = 20 μm). Data are represented as means ± SEM. * p < 0.05, ** p < 0.01 compared with the 24 h-control group. ^# p < 0.05, ^## p < 0.01 compared with the 48 h-control group.