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. 2019 Jul 24;8(8):769. doi: 10.3390/cells8080769

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Droplet digital PCR analysis. Examples of ddPCR plot for two metastatic colorectal cancer (mCRC) patients (n.7 and n.13) and respective fractional abundance of the mutated KRAS allele at the 3 time point: Patient n.7 = G12C KRAS mutation (upper panel) and Patient n.13 = A146T KRAS mutation (lower panel). Mutants are clustered in the upper left corner with high Fluorescein (FAM) fluorescent intensities (blue), wild-type is clustered in lower right corner with high Hexachloro-fluorescein (HEX) fluorescent intensities (green) while mutant plus wild-type sequences are clustered in the upper right corner (orange). The empty droplets are represented in black.