Figure 2.

Representative example of a Western Blot on samples of Gsdme KO and WT mice. Western Blotting was performed with a primary rabbit anti-DFNA5/GSDME-N-Terminal antibody (ab215191) and a secondary Goat Anti-Rabbit IgG (H+L)-HRP Conjugate (#1706515). Clear GSDME protein bands ($~$57 kDa) were seen, both in the brain and colorectal tissue of WT mice. GSDME could not be detected in Gsdme KO brain or colorectal tissues. In all samples, clear bands for β-actin could be seen ($~$42 kDa). WT = wild type mice; KO = Gsdme knockout mice; B = brain tissue; C = colorectal tissue.