Table 1.
Overview of all lesions scored in the large intestine of AOM-treated mice (all timepoints combined) and in the small intestine of Apc1638N/+ mice.
| Lesion | Chemical Experiment—Large Intestine | Genetic Experiment—Small Intestine | ||||||
|---|---|---|---|---|---|---|---|---|
| Gsdme KO (n = 46) | WT (n = 54) | Δ (%) | p-Value | Apc1638N/+ Gsdme KO (n = 13) | Apc1638N/+ Gsdme WT (n = 24) | Δ (%) | p-Value | |
| OVERALL | ||||||||
| moderate mucosal inflammation | 19 | 20 | 4.3 | 0.73 | 3 | 16 | −43.6 | 0.010 |
| marked mucosal inflammation | 4 | 1 | 6.8 | 0.014 | 0 | 0 | 0.0 | - |
| glandular cyst | 46 | 54 | 0.0 | - | 0 | 0 | 0.0 | - |
| typical hyperplasia | 21 | 26 | −2.4 | 0.70 | 1 | 9 | −29.8 | 0.034 |
| atypical hyperplasia | 18 | 13 | 15.0 | 0.081 | 5 | 9 | 1.0 | 0.90 |
| adenoma | 3 | 1 | 4.6 | 0.57 | 0 | 0 | 0.0 | - |
| adenocarcinoma | 20 | 25 | −2.8 | 0.67 | 10 | 16 | 10.2 | 0.55 |
| proliferative change | 34 | 36 | 7.2 | 0.22 | 11 | 23 | −11.2 | 0.21 |
| PROXIMAL | ||||||||
| moderate mucosal inflammation | 6 | 4 | 5.6 | 0.31 | 2 | 9 | −22.1 | 0.13 |
| marked mucosal inflammation | 0 | 0 | 0.0 | - | 0 | 0 | 0.0 | - |
| glandular cyst | 25 | 43 | −25.3 | 0.043 | 0 | 0 | 0.0 | - |
| typical hyperplasia | 0 | 0 | 0.0 | - | 0 | 7 | −29.2 | 0.0077 |
| atypical hyperplasia | 0 | 0 | 0.0 | - | 3 | 6 | −1.9 | 0.97 |
| adenoma | 0 | 0 | 0.0 | - | 0 | 0 | 0.00 | - |
| adenocarcinoma | 0 | 0 | 0.0 | - | 8 | 13 | 7.3 | 0.74 |
| proliferative change | 0 | 0 | 0.0 | - | 10 | 22 | −14.8 | 0.17 |
| MID 1 | ||||||||
| moderate mucosal inflammation | 9 | 7 | 6.6 | 0.37 | 1 | 5 | −13.1 | 0.30 |
| marked mucosal inflammation | 1 | 0 | 2.2 | 0.053 | 0 | 0 | 0.0 | - |
| glandular cyst | 21 | 47 | −41.3 | 0.00038 | 0 | 0 | 0.0 | - |
| typical hyperplasia | 2 | 4 | −3.1 | 0.64 | 0 | 2 | −8.3 | 0.19 |
| atypical hyperplasia | 5 | 0 | 10.9 | 0.023 | 1 | 5 | −13.1 | 0.29 |
| adenoma | 0 | 0 | 0.0 | - | 0 | 0 | 0.0 | - |
| adenocarcinoma | 1 | 2 | −1.5 | 0.69 | 5 | 8 | 5.2 | 0.77 |
| proliferative change | 8 | 6 | 6.3 | 0.53 | 6 | 12 | −3.8 | 0.83 |
| MID 2 | ||||||||
| moderate mucosal inflammation | 12 | 12 | 3.9 | 0.57 | 0 | 7 | −29.2 | 0.0088 |
| marked mucosal inflammation | 3 | 0 | 6.5 | 0.019 | 0 | 0 | 0.0 | - |
| glandular cyst | 35 | 44 | −5.4 | 0.79 | 0 | 0 | 0.0 | - |
| typical hyperplasia | 8 | 12 | −4.8 | 0.25 | 0 | 2 | −8.3 | 0.19 |
| atypical hyperplasia | 9 | 6 | 8.5 | 0.27 | 2 | 1 | 11.2 | 0.24 |
| adenoma | 1 | 0 | 2.2 | 0.50 | 0 | 0 | 0.0 | - |
| adenocarcinoma | 12 | 13 | 2.0 | 0.68 | 0 | 4 | −16.7 | 0.049 |
| proliferative change | 24 | 21 | 13.3 | 0.20 | 2 | 6 | −9.6 | 0.48 |
| DISTAL | ||||||||
| moderate mucosal inflammation | 7 | 11 | −5.2 | 0.51 | 0 | 3 | −12.5 | 0.098 |
| marked mucosal inflammation | 0 | 1 | −1.9 | 0.49 | 0 | 0 | 0.0 | - |
| glandular cyst | 43 | 53 | −4.7 | 0.57 | 0 | 0 | 0.0 | - |
| typical hyperplasia | 18 | 19 | 3.9 | 0.19 | 1 | 0 | 7.7 | 0.15 |
| atypical hyperplasia | 7 | 10 | −3.3 | 0.95 | 0 | 0 | 0.0 | - |
| adenoma | 2 | 1 | 2.5 | 0.72 | 0 | 0 | 0.0 | - |
| adenocarcinoma | 13 | 18 | −5.1 | 0.46 | 3 | 3 | 10.6 | 0.43 |
| proliferative change | 28 | 32 | 1.6 | 0.63 | 4 | 3 | 18.3 | 0.20 |
The number of mice with at least one specific lesion throughout the whole large intestine (chemical experiment) or small intestine (genetic experiment) (overall), or in one specific part of the large intestine/small intestine (proximal, mid 1, mid 2, and distal), in the Gsdme KO and WT group is indicated. The p-value indicates the significance for a difference between Gsdme KO and WT mice, accounting for sex and age. Proliferative change includes typical hyperplasia, atypical hyperplasia, adenoma, and/or adenocarcinoma.