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. 2019 Jul 30;8(8):794. doi: 10.3390/cells8080794

Figure 2.

Figure 2

Early p53 depletion in CRC cells and impact on p53-dependent gene expression. (A) CRC cells with wildtype p53 (HCT116 and RKO) and mutant p53 (HT29) were treated with increasing doses of LA for 24 h as indicated. EtOH was included as solvent control (0 µM). Depletion of p53 was monitored using western blot analysis. Hsp90 was visualized as loading control. (B) Densitometric analysis of data presented in A. p53 levels were normalized to Hsp90. Data is presented as mean + SEM (n = 3). * p < 0.05; ** p < 0.01. (C) Gene expression of p53 targets genes (MDM2, GADD45a, p21, FASR, NOXA, and PUMA) in HCT116 cells treated with LA for 24 h determined by qPCR analysis. EtOH served as solvent control (0 µM LA), while 5-FU was used as positive control. Gene expression levels are normalized to ACTB and GAPDH. Data is presented as mean + SEM (n = 3), except for FASR analysis (n = 2).