Table 4.
Immunocompromised mouse strains for PDX.
| Mouse Strain | Phenotype | Advantage | Disadvantage/Consideration | Success Rate of PDX |
|---|---|---|---|---|
| Nude | No thymus, no coat of hair |
Well characterized, easy to detect s.c. tumor |
Functional B and NK cells, increased T cell leakage with age |
Low |
| SCID | No mature T and B cells | Better engraftment compared with nude | Functional NK cell, leakage of T cells, radiosensitive |
Low |
| SCID/Beige | No mature T and B cells, impaired Mφ and NK function |
Better engraftment compared with SCID | Leakage of T cells, radiosensitive |
Moderate |
| NOD/SCID | No mature T and B cells Impaired NK function Impaired Mφ & DC |
Better engraftment | Spontaneous lymphoma Short life span (av. 36wks) Radiosensitive |
Moderate |
| NOG/NSG/NOJ | No mature T and B cells, no NK cells, impaired Mφ and DC |
Excellent engraftment of PDX including hematopoietic malignancies | Need strict SPF conditions, breeding is not easy, expensive |
High |
| BALB/c Rag2null/IL2Rγnull (BRG) Rag-2 null/Jak3 null (BRJ) |
No mature T and B cells, no NK cells |
Excellent engraftment of PDX, resistant to stress, easy breeding, radio resistant |
High |
NK: natural killer cells, Mφ: macrophages, DCs: dendritic cells, NOG/NSG: NOD/SCID/IL2Rγnull, NOJ: NOD/SCID/Jak3null, s.c.: subcutaneous.