Figure 5.

Combinatorial targeting of both EGFR and MAP kinase signaling pathways show synergistic effects against colorectal cancer cell lines. (A–D) Colony formation assay was performed with LS174T (A), DLD1 (B), DiFi (C) and WiDr (D) cells following treatment with either CTX (50 μg/mL or 10 μg/mL for DiFi) or selumetinib (0.1 μM) or together. The bar graph depicts the relative number of colonies formed by these cells following normalization to that of the same cells treated with PBS (control) (n = 3, mean + SD). Representative photomicrographs of colonies formed in soft agar are shown.