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. 2019 Aug 19;8(8):934. doi: 10.3390/cells8080934

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Comparison of the differentiation degree of microtissues from one representative donor after 4 and 8 weeks of cultivation using cryosections. (Upper rows) Histological analyses visualizing glycosaminoglycans (Alcian Blue: Blue, Safranin O: Red) and cell nuclei (Alcian Blue: Pink, Safranin O: Cyan). Scale bars: 200 µm. (Lower rows) Immunohistochemical analyses of cartilage-specific markers. Red: Collagen type I (Coll I), collagen type II (Coll II), proteoglycans (PG), and Sox9, respectively. Blue: Cell nuclei. Scale bars: 50 µm.