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. 2019 Aug 12;8(8):879. doi: 10.3390/cells8080879

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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iPla2β (calcium-independent phospholipase A2) deficiency during MCD (methionine- and choline-deficient) induces liver fibrosis without affecting steatosis. Female WT (wild-type) and iPla2β^−/− mice were fed with chow or MCD diet for 5 weeks. (A) Body and % liver weights. (B) Serum ALT, glucose, and total fatty acids determined by GC-MS. (C) Liver triglycerides (TG) and non-esterified or free fatty acids (FFA). (D) Liver PC (phosphatidylcholine), saturated LPC (lysophosphatidylcholine), and saturated LPE (lysophosphatidylethanolamine) determined by LC-MS/MS. (E) Liver slides were subjected to H&E (hematoxylin and eosin), Sirius-Red, and α-SMA IHC (immunohistochemistry) staining. (F) Expression of α-SMA, TGF-β1 (transforming growth factor-β1), collagen3α1, and collagen4α1 mRNA. (G) Expression of MCP-1 (monocyte chemoattractant protein-1) and CCR2 (CC motif chemokine receptor 2) mRNA. Data are ± SEM, N = 4–7; * p < 0.05 between indicated groups.