. 2019 Aug 20;8(8):939. doi: 10.3390/cells8080939

Table 1.

A summary of eTreg-related transcription factor expression and functions.

Name	Expression	Function	Target	Upstream Regulator	Ref.
IRF4	eTreg	Regulates eTreg differentiation	ICOS, Blimp1, FGL2, ST2, GATA3, CCR8, CTLA4, KLRG1, IL-10, CCR6	TCR+CD28+IL-2	[16,53,54]
BATF	eTreg	Promotes expression of eTreg-related genes	ICOS, GATA3, ST2, KLRG1, CCR4, CCR6	TCR+CD28+IL-2	[17,54,55]
JunB	eTreg	Promotes expression of eTreg-related genes	ICOS, FGL2, CTLA4, TIGIT, KLRG1	TCR+CD28+IL-2	[23,56,57]
Blimp-1	eTreg	Promotes expression of eTreg-related genes	IL-10, KLRG1, Ebi3, CCR6, BCL2	TCR+CD28+IL-2	[58]
Myb	eTreg	Promotes expression of eTreg-related genesin tTreg	ICOS, TIGIT, KLRG1, BCL2, MYC	TCR+CD28+IL-2	[22]
RelA	All Tregs	Promotes expression of eTreg-related genes	TIGIT, ST2, KLRG1, CD103, CD30	TNF-α, GITR	[59,60]
c-Rel	All Tregs	Promotes expression of eTreg-related genes	TIGIT, ST2, ICOS, EBI3,Blimp-1,IL-10	Unknown	[59,61]
Id2	eTreg	Suppresses Tfr differentiation	CXCR5, IL-10, HIF1-α, IKZF3, Myb, IL-10 rα, E2F2	TCR+CD28+IL-2	[62,63,64]
Id3	cTreg, CD44^lo eTreg	Suppresses Tfr differentiation	CXCR5, IL-10, HIF1-α, IKZF3, Myb, IL-10 rα, E2F2	TCR+CD28+IL-2- Erk,PI3K/mTOR	[62,63]
E2A/ HEB	eTreg	Suppresses eTreg differentiation	ICOS, IRF4, CD103, KLRG1, RORγt	TCR+CD28	[65]
TCF1/ LEF1	cTreg, CD44^lo eTreg	Regulates Tfr differentiationSuppresses expression of eTreg-related genes?	CD44, ICOS, TIGIT, IRF4, GATA3, Blimp-1, T-bet, Bcl6, CXCR5	Unknown	[66,67]
Foxo1	All Treg	Maintains expression of cTreg-related genes	CD62L, CCR7, Bim, GzmB	TCR+CD28-Akt	[18,68]
T-bet	eTreg, Tissue Tregs	Regulates migration to Th1-inflammatory sites	CXCR3	IFNγ-STAT1	[45,69]
GATA3	Skin and Intestinal tTreg	Maintains Treg homeostasis Suppresses skin inflammation	ST2, Foxp3	TCR, IL-4, IL-33	[37,70,71]
RORγt	Intestinal pTreg	Regulates migration to intestinal tissue	CCR6	IL-6/IL-23-STAT3, Microbiota	[35,36,72]
BCL6	Follicular Treg	Regulates Tfr differentiation	CXCR5, PD-1	IL-21, IL-6	[73,74]
c-Maf	eTreg	Regulates RORγτ⁺ Treg and Tfr differentiations	RORγt, CXCR5, IL-10	TCR+CD28+IL-2, STAT3, Microbiota, Notch1/2	[75,76,77]
STAT3	All Tregs	Regulates RORγt⁺ Treg and Tfr differentiation	TCF1, RORγt	IL-6,IL-23,IL-21	[35,74,75,78,79]
PPARγ	VAT-Treg	Regulates VAT-Treg differentiation	CCR1, PCYT1A, DGAT1, IL-10	TCR,IL-33, Adipose tissue-derived factor?	[32,34,44,54]
RORα	Skin and Colon Tregs	Promotes expression of eTreg-related genes	CCR6, CD73, DR3	Unknown	[52,80]