Figure 3.

Loss of Elf3 preserves the mesenchymal state. NMuMG cells recovering from TGFβ3 treatment were transfected with either control or Elf3 targeting siRNA, after 72 h, cells were collected, RNA was isolated, and changes in gene expression were measured by qPCR for EMT/MET relevant genes (A) and mesenchymal markers (B). (C) Immunoblot showing ELF3 and E-cadherin protein levels in response to Elf3 silencing by siRNAs. (D) Summary of the wound healing assay results following Elf3 silencing measured as the distance between the edges of migrating cells. Results represent the averages of at least three independent experiments in triplicates. Paired Student’s t-test was used to calculate statistical significance. * p-value < 0.05; ** p-value < 0.01; *** p-value < 0.001; **** p-value < 0.0001.