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. 2019 Aug 19;8(8):935. doi: 10.3390/cells8080935

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Inhibition of miR-21 reduced the effect of conditioned medium on ameliorating myocardial infiltration of CD68+ macrophages. (A) Immunofluorescent staining to detect the distribution of CD68+ macrophages in sham control myocardial section. (B) Immunofluorescent staining to detect the distribution of CD68+ macrophages in myocardial section from rats with AMI induction. (C) Immunofluorescent staining to detect the distribution of CD68+ macrophages in myocardial section from rats with AMI induction and injection of conditioned medium derived from OGD-treated cardiomyocytes. (D) Immunofluorescent staining to detect the distribution of CD68+ macrophages in myocardial section from rats with AMI induction and injection of conditioned medium derived from OGD-treated cardiomyocytes with anti-miR-21 pre-transfection. (E) Calculation of CD68+ cells in myocardium. SC, sham control. AMI, acute myocardial infarction. CM, conditioned medium derived from OGD-treated cardiomyocyte. Anti-miR-21-CM, conditioned medium derived from cardiomyocyte pre-transfected with anti-miR-21 (20 nM). Groups with different symbols (*, †, ‡), p < 0.05. n = 8 for each group. Scale bar in left lower corner indicates 50 μm.